Vol 5, No 1 (2017)


Cover Page
Spaceflight and the Antibody Repertoire. This issue features a paper detailing the bioinformatic workflow developed for assessing the effects of spaceflight on the antibody repertoire (p 2). Top right: Animal Enclosure Module (AEM) hardware was validated on the Rodent Research-1 (RR1) mission. High throughput sequencing (HTS) data sets of RR1 animals were made available by NASA GeneLab and spleen tissues were made available by NASA Ames. These were mined for antibody repertoire assessment. Bottom right: Antibodies are heterodimers of heavy (IgH) and light immunoglobulin chains that are encoded on separate loci. A diagram of the IgH locus is pictured. Multiple copies of Variable- (V), Diversity- (D), and Joining- (J) gene segments exist within the germline IgH locus. Somatic recombination at the IgH locus during early B-cell development yields immunoglobulin sequences with one V-D-J-gene segment combination, collectively resulting in a repertoire of antibody specificities that can bind to antigens for host defense. Background image: The antibody repertoire can be characterized by assessing gene segment usage in HTS data sets. Image credits: AEM: NASA, picture of C57BL/6 Mouse: Chapes laboratory (not to scale).


ISSN: 2332-7774