Enzymatic Catalysis in Organic Media: Fundamentals and Selected Applications
The use of enzymes in organic solvents significantly extends conventional aqueous-based biocatalysis. This field is reviewed with respect to the salient factors that govern enzymatic catalysis in non-aqueous media. Specific examples of peroxidase catalysis in organic media are discussed (e.g., phenolic polymerizations and enzymic temperature abuse sensors). Given that membranous parts of a cell are highly non-aqueous in character, the study of enzymes in poorly hydrated media may provide clues as to the function of membrane-bound enzymes and their response to different gravitational forces.